Chemoenzymatic Site-Specific Labeling of Influenza Glycoproteins as a Tool to Observe Virus Budding in Real Time
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Popp-2012-Chemoenzymatic Site-.pdf
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1.7 MB
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Checksum (MD5)
23e27ed0fe7746f2cf1f732bdbebb97a
Author(s)
Karssemeijer, Roos A.
Ploegh, Hidde
Popp, Maximilian W.L.
Date Issued
March 2012
Journal
PLoS Pathogens
Publisher
Public Library of Science
Citation
Popp, Maximilian Wei-Lin, Roos A. Karssemeijer, and Hidde L. Ploegh. “Chemoenzymatic Site-Specific Labeling of Influenza Glycoproteins as a Tool to Observe Virus Budding in Real Time.” Ed. Yoshihiro Kawaoka. PLoS Pathogens 8.3 (2012): e1002604.
Version
Final published version
Abstract
The influenza virus uses the hemagglutinin (HA) and neuraminidase (NA) glycoproteins to interact with and infect host cells. While biochemical and microscopic methods allow examination of the early steps in flu infection, the genesis of progeny virions has been more difficult to follow, mainly because of difficulties inherent in fluorescent labeling of flu proteins in a manner compatible with live cell imaging. We here apply sortagging as a chemoenzymatic approach to label genetically modified but infectious flu and track the flu glycoproteins during the course of infection. This method cleanly distinguishes influenza glycoproteins from host glycoproteins and so can be used to assess the behavior of HA or NA biochemically and to observe the flu glycoproteins directly by live cell imaging.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1371/journal.ppat.1002604
