Assembly of RNA polymerase II transcription initiation complexes
Name
nihms-1825455.pdf
Description
Accepted version
Size
1.58 MB
Format
Adobe PDF
Checksum (MD5)
5e834932858ab1532f91b20710c5eb08
Author(s)
Farnung, Lucas
Vos, Seychelle M
Date Issued
2022
Journal
Current Opinion in Structural Biology
Publisher
Elsevier BV
Citation
Farnung, Lucas and Vos, Seychelle M. 2022. "Assembly of RNA polymerase II transcription initiation complexes." Current Opinion in Structural Biology, 73.
Version
Author's final manuscript
Abstract
The first step of eukaryotic gene expression is the assembly of RNA polymerase II and general transcription factors on promoter DNA. This highly regulated process involves ∼80 different proteins that together form the preinitiation complex (PIC). Decades of work have gone into understanding PIC assembly using biochemical and structural approaches. These efforts have yielded significant but partial descriptions of PIC assembly. Over the past few years, cryo-electron microscopy has provided the first high-resolution structures of the near-complete mammalian PIC assembly. These structures have revealed that PIC assembly is a highly dynamic process. This review will summarize recent structural findings and discuss their implications for understanding cell type-specific gene expression.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.SBI.2022.102335
