Identification and localization of a neuron-specific isoform of TAF1 in rat brain: implications for neuropathology of DYT3 dystonia
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Graybiel_Identification and localization.pdf
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Author(s)
Sako, W.
Morigaki, R.
Kaji, R.
Tooyama, I.
Okita, S.
Kitazato, K.
Nagahiro, S.
Goto, S.
Graybiel, Ann M.
Date Issued
May 2011
Journal
Neuroscience
Publisher
Elsevier
Citation
Sako, W., R. Morigaki, R. Kaji, I. Tooyama, S. Okita, K. Kitazato, S. Nagahiro, A.M. Graybiel, and S. Goto. “Identification and Localization of a Neuron-Specific Isoform of TAF1 in Rat Brain: Implications for Neuropathology of DYT3 Dystonia.” Neuroscience 189 (August 2011): 100–107.
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Author's final manuscript
Abstract
The neuron-specific isoform of the TAF1 gene (N-TAF1) is thought to be involved in the pathogenesis of DYT3 dystonia, which leads to progressive neurodegeneration in the striatum. To determine the expression pattern of N-TAF1 transcripts, we developed a specific monoclonal antibody against the N-TAF1 protein. Here we show that in the rat brain, N-TAF1 protein appears as a nuclear protein within subsets of neurons in multiple brain regions. Of particular interest is that in the striatum, the nuclei possessing N-TAF1 protein are largely within medium spiny neurons, and they are distributed preferentially, though not exclusively, in the striosome compartment. The compartmental preference and cell type-selective distribution of N-TAF1 protein in the striatum are strikingly similar to the patterns of neuronal loss in the striatum of DYT3 patients. Our findings suggest that the distribution of N-TAF1 protein could represent a key molecular characteristic contributing to the pattern of striatal degeneration in DYT3 dystonia.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
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DOI of Published Version
https://doi.org/10.1016/j.neuroscience.2011.05.031
