Chromatin Dynamics and the RNA Exosome Function in Concert to Regulate Transcriptional Homeostasis
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Rege-2015-Chromatin Dynamics a.pdf
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Author(s)
Rege, Mayuri
Subramanian, Vidya
Zhu, Chenchen
Hsieh, Tsung-Han S.
Weiner, Assaf
Friedman, Nir
Steinmetz, Lars M.
Rando, Oliver J.
Peterson, Craig L.
Clauder-Munster, Sandra
Date Issued
November 2015
Journal
Cell Reports
Publisher
Elsevier
Citation
Rege, Mayuri, Vidya Subramanian, Chenchen Zhu, Tsung-Han S. Hsieh, Assaf Weiner, Nir Friedman, Sandra Clauder-Munster, et al. “Chromatin Dynamics and the RNA Exosome Function in Concert to Regulate Transcriptional Homeostasis.” Cell Reports 13, no. 8 (November 2015): 1610–1622.
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Author's final manuscript
Abstract
The histone variant H2A.Z is a hallmark of nucleosomes flanking promoters of protein-coding genes and is often found in nucleosomes that carry lysine 56-acetylated histone H3 (H3-K56Ac), a mark that promotes replication-independent nucleosome turnover. Here, we find that H3-K56Ac promotes RNA polymerase II occupancy at many protein-coding and noncoding loci, yet neither H3-K56Ac nor H2A.Z has a significant impact on steady-state mRNA levels in yeast. Instead, broad effects of H3-K56Ac or H2A.Z on RNA levels are revealed only in the absence of the nuclear RNA exosome. H2A.Z is also necessary for the expression of divergent, promoter-proximal noncoding RNAs (ncRNAs) in mouse embryonic stem cells. Finally, we show that H2A.Z functions with H3-K56Ac to facilitate formation of chromosome interaction domains (CIDs). Our study suggests that H2A.Z and H3-K56Ac work in concert with the RNA exosome to control mRNA and ncRNA expression, perhaps in part by regulating higher-order chromatin structures.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Biology
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2015.10.030
