MyD88 signalling in colonic mononuclear phagocytes drives colitis in IL-10-deficient mice
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Fox_MyD88 signalling.pdf
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Author(s)
Hoshi, Namiko
Schenten, Dominik
Nish, Simone A.
Walther, Zenta
Gagliani, Nicola
Flavell, Richard A.
Reizis, Boris
Shen, Zeli
Fox, James G.
Iwasaki, Akiko
Date Issued
October 2012
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Hoshi, Namiko, Dominik Schenten, Simone A. Nish, Zenta Walther, Nicola Gagliani, Richard A. Flavell, Boris Reizis, et al. “MyD88 Signalling in Colonic Mononuclear Phagocytes Drives Colitis in IL-10-Deficient Mice.” Nature Communications 3 (October 9, 2012): 1120.
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Author's final manuscript
Abstract
Commensal bacterial sensing by Toll-like receptors is critical for maintaining intestinal homeostasis, but can lead to colitis in the absence of interleukin-10. Although Toll-like receptors are expressed in multiple cell types in the colon, the cell type(s) responsible for the development of colitis are currently unknown. Here we generated mice that are selectively deficient in MyD88 in various cellular compartments in an interleukin-10[superscript −/−] setting. Although epithelial expression of MyD88 was dispensable, MyD88 expression in the mononuclear phagocyte compartment was required for colitis development. Specifically, phenotypically distinct populations of colonic mononuclear phagocytes expressed high levels of interleukin-1β, interleukin-23 and interleukin-6, and promoted T-helper 17 responses in the absence of interleukin-10. Thus, gut bacterial sensing through MyD88 in mononuclear phagocytes drives inflammatory bowel disease when unopposed by interleukin-10.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Division of Comparative Medicine
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DOI of Published Version
https://doi.org/10.1038/ncomms2113
