A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria
Name
44216_3_merged_1545417160.pdf
Description
Accepted version
Size
22.93 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Stokes, Jonathan
Gutierrez, Arnaud
Lopatkin, Allison J.
Andrews, Ian W.
French, Shawn
Matic, Ivan
Brown, Eric D.
Collins, James J.
Date Issued
March 2019
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Stokes, J.M. et al. A multiplexable assay for screening antibiotic lethality against drug-tolerant bacteria. Nature Methods 16, 4 (March 2019): 303–306 © 2019 The Author(s)
Version
Author's final manuscript
Abstract
Antibiotic screens typically rely on growth inhibition to characterize compound bioactivity—an approach that cannot be used to assess the bactericidal activity of antibiotics against bacteria in drug-tolerant states. To address this limitation, we developed a multiplexed assay that uses metabolism-sensitive staining to report on the killing of antibiotic-tolerant bacteria. This method can be used with diverse bacterial species and applied to genome-scale investigations to identify therapeutic targets against tolerant pathogens.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biological Engineering
Harvard University--MIT Division of Health Sciences and Technology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/s41592-019-0333-y
