Expansion microscopy
Name
Boyden_Expansion microscopy.pdf
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1013.63 KB
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Adobe PDF
Checksum (MD5)
44ec225c2e54dffc6e313cfdc8901fc5
Author(s)
Chen, Fei
Tillberg, Paul W.
Boyden, Edward
Date Issued
January 2015
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Chen, Fei, Paul W. Tillberg, and Edward S. Boyden. “Expansion Microscopy.” Science 347, no. 6221 (January 15, 2015): 543–548.
Version
Author's final manuscript
Abstract
In optical microscopy, fine structural details are resolved by using refraction to magnify images of a specimen. We discovered that by synthesizing a swellable polymer network within a specimen, it can be physically expanded, resulting in physical magnification. By covalently anchoring specific labels located within the specimen directly to the polymer network, labels spaced closer than the optical diffraction limit can be isotropically separated and optically resolved, a process we call expansion microscopy (ExM). Thus, this process can be used to perform scalable superresolution microscopy with diffraction-limited microscopes. We demonstrate ExM with apparent ~70-nanometer lateral resolution in both cultured cells and brain tissue, performing three-color superresolution imaging of ~107 cubic micrometers of the mouse hippocampus with a conventional confocal microscope.
Description
Available in PMC 2015 July 30.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Massachusetts Institute of Technology. Center for Neurobiological Engineering
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1126/science.1260088
