Simultaneous imaging of GFP, CFP and collagen in tumors in vivousing multiphoton microscopy
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1472-6750-5-14.pdf
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Checksum (MD5)
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Author(s)
Sahai, Erik
Wyckoff, Jeffrey
Philippar, Ulrike
Segall, Jeffrey E
Gertler, Frank
Condeelis, John S.
Date Issued
May 2005
Journal
BMC Biotechnology
Publisher
BioMed Central Ltd
Citation
BMC Biotechnology. 2005 May 23;5(1):14
Version
Final published version
Abstract
Background: The development of multiphoton laser scanning microscopy has greatly facilitated the imaging of living tissues. However, the use of genetically encoded fluorescent proteins to distinguish different cell types in living animals has not been described at single cell resolution using multiphoton microscopy. Results: Here we describe a method for the simultaneous imaging, by multiphoton microscopy, of Green Fluorescent Protein, Cyan Fluorescent Protein and collagen in vivo in living tumors. This novel method enables: 1) the simultaneous visualization of overall cell shape and sub-cellular structures such as the plasma membrane or proteins of interest in cells inside living animals, 2) direct comparison of the behavior of single cells from different cell lines in the same microenvironment in vivo. Conclusion: Using this multi-fluor, multiphoton technique, we demonstrate that motility and metastatic differences between carcinoma cells of differing metastatic potential can be imaged in the same animal simultaneously at sub-cellular resolution.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1186/1472-6750-5-14
