Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition
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Bartel_Widespread changes.pdf
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Author(s)
Kronja, Iva
Yuan, Bingbing
Dzeyk, Kristina
Krijgsveld, Jeroen
Eichhorn, Stephen William
Bartel, David
Orr-Weaver, Terry L.
Orr-Weaver, Terry
Date Issued
May 2014
Journal
Cell Reports
Publisher
Elsevier
Citation
Kronja, Iva, Bingbing Yuan, Stephen W. Eichhorn, Kristina Dzeyk, Jeroen Krijgsveld, David P. Bartel, and Terry L. Orr-Weaver. “Widespread Changes in the Posttranscriptional Landscape at the Drosophila Oocyte-to-Embryo Transition.” Cell Reports 7, no. 5 (June 2014): 1495–1508.
Version
Final published version
Abstract
The oocyte-to-embryo transition marks the onset of development. The initial phase of this profound change from the differentiated oocyte to the totipotent embryo occurs in the absence of both transcription and mRNA degradation. Here we combine global polysome profiling, ribosome-footprint profiling, and quantitative mass spectrometry in a comprehensive approach to delineate the translational and proteomic changes that occur during this important transition in Drosophila. Our results show that PNG kinase is a critical regulator of the extensive changes in the translatome, acting uniquely at this developmental window. Analysis of the proteome in png mutants provided insights into the contributions of translation to changes in protein levels, revealing a compensatory dynamic between translation and protein turnover during proteome remodeling at the return to totipotency. The proteome changes additionally suggested regulators of meiosis and early embryogenesis, including the conserved H3K4 demethylase LID, which we demonstrated is required during this period despite transcriptional inactivity.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution-NonCommercial-No Derivative Works 3.0 Unported
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DOI of Published Version
https://doi.org/10.1016/j.celrep.2014.05.002
