Advances in Chromatin and Chromosome Research: Perspectives from Multiple Fields
Name
nihms-1642195.pdf
Description
Accepted version
Size
2.13 MB
Format
Adobe PDF
Checksum (MD5)
77363361e0095a6fc044a5418c290ad4
Author(s)
Agbleke, Andrews Akwasi
Amitai, Assaf
Buenrostro, Jason D
Chakrabarti, Aditi
Chu, Lingluo
Hansen, Anders S
Koenig, Kristen M
Labade, Ajay S
Liu, Sirui
Nozaki, Tadasu
Date Issued
2020
Journal
Molecular Cell
Publisher
Elsevier BV
Version
Author's final manuscript
Abstract
© 2020 Elsevier Inc. Nucleosomes package genomic DNA into chromatin. By regulating DNA access for transcription, replication, DNA repair, and epigenetic modification, chromatin forms the nexus of most nuclear processes. In addition, dynamic organization of chromatin underlies both regulation of gene expression and evolution of chromosomes into individualized sister objects, which can segregate cleanly to different daughter cells at anaphase. This collaborative review shines a spotlight on technologies that will be crucial to interrogate key questions in chromatin and chromosome biology including state-of-the-art microscopy techniques, tools to physically manipulate chromatin, single-cell methods to measure chromatin accessibility, computational imaging with neural networks and analytical tools to interpret chromatin structure and dynamics. In addition, this review provides perspectives on how these tools can be applied to specific research fields such as genome stability and developmental biology and to test concepts such as phase separation of chromatin.
MIT Department
Massachusetts Institute of Technology. Institute for Medical Engineering & Science
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.MOLCEL.2020.07.003
