The rational design of nitric oxide selectivity in single-walled carbon nanotube near infrared fluorescence sensors for biological detection
Name
stranorationaldesignofnitricoxideselectivity.pdf
Size
810.68 KB
Format
Adobe PDF
Checksum (MD5)
8d5f8799f74333d06d1018fab093340f
Author(s)
Kim, Jong-Ho
Heller, Daniel A.
Jin, Hong
Barone, Paul W.
Song, Changsik
Zhang, Jingqing
Trudel, Laura J.
Wogan, Gerald N.
Tannenbaum, Steven Robert
Strano, Michael S.
Date Issued
August 2009
Journal
Nature Chemistry
Publisher
Nature Publishing Group
Citation
Kim, Jong-Ho et al. “The rational design of nitric oxide selectivity in single-walled carbon nanotube near-infrared fluorescence sensors for biological detection.” Nat Chem 1.6 (2009): 473-481.
Version
Author's final manuscript
Abstract
A major challenge in the synthesis of nanotube or nanowire sensors is imparting selective analyte binding through means other than covalent linkages which compromise electronic and optical properties. We synthesize a 3,4-diaminophenyl-functionalized dextran (DAP-dex) wrapping for single-walled carbon nanotubes (SWNT) that imparts rapid and selective fluorescence detection of NO, a messenger for biological signaling. The near infrared (nIR) fluorescence of SWNT[ subscript DAP-dex] is immediately and directly bleached by NO, but not by other reactive nitrogen and oxygen species. This bleaching is reversible and shown to be caused by electron transfer from the top of the valence band of SWNT to the lowest unoccupied molecular orbital (LUMO) of NO. The resulting optical sensor is capable of real-time and spatially resolved detection of NO produced by stimulating NO synthase (iNOS) in macrophage cells. We also demonstrate the potential of the optical sensor for in-vivo detection of NO in a mouse model.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
http://dx.doi.org/10.1038/nchem.332
