Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release
Name
nihms-1515668.pdf
Description
Accepted version
Size
663.96 KB
Format
Adobe PDF
Checksum (MD5)
d47b62862b2ff1a913b9e778176908f8
Author(s)
Harris, Kathryn P.
Littleton, J. Troy
Stewart, Bryan A.
Date Issued
September 2018
Journal
Journal of Neurogenetics
Publisher
Informa UK Limited
Citation
Harris, Kathryn P. et al. "Postsynaptic Syntaxin 4 negatively regulates the efficiency of neurotransmitter release." Journal of Neurogenetics 32, 3 (September 2018): 221-229 © 2018 Informa UK Limited
Version
Author's final manuscript
Abstract
Signaling from the postsynaptic compartment regulates multiple aspects of synaptic development and function. Syntaxin 4 (Syx4) is a plasma membrane t-SNARE that promotes the growth and plasticity of Drosophila neuromuscular junctions (NMJs) by regulating the localization of key synaptic proteins in the postsynaptic compartment. Here, we describe electrophysiological analyses and report that loss of Syx4 leads to enhanced neurotransmitter release, despite a decrease in the number of active zones. We describe a requirement for postsynaptic Syx4 in regulating several presynaptic parameters, including Ca2+ cooperativity and the abundance of the presynaptic calcium channel Cacophony (Cac) at active zones. These findings indicate Syx4 negatively regulates presynaptic neurotransmitter release through a retrograde signaling mechanism from the postsynaptic compartment.
MIT Department
Picower Institute for Learning and Memory
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1080/01677063.2018.1501372
