High-throughput quantitation of inorganic nanoparticle biodistribution at the single-cell level using mass cytometry
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high-throughput quantitation.pdf
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Author(s)
Bekdemir, Ahmet
Yang, Yu-Sang Sabrina
Atukorale, Prabhani Upeka
Moynihan, Kelly Dare
Rakhra, Kavya
Tang, Li
Stellacci, Francesco
Irvine, Darrell J
Date Issued
January 2017
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Yang, Yu-Sang Sabrina, Prabhani U. Atukorale, Kelly D. Moynihan, Ahmet Bekdemir, Kavya Rakhra, Li Tang, Francesco Stellacci, and Darrell J. Irvine. “High-Throughput Quantitation of Inorganic Nanoparticle Biodistribution at the Single-Cell Level Using Mass Cytometry.” Nature Communications 8 (January 17, 2017): 14069.
Version
Final published version
Abstract
Inorganic nanoparticles (NPs) are studied as drug carriers, radiosensitizers and imaging agents, and characterizing nanoparticle biodistribution is essential for evaluating their efficacy and safety. Tracking NPs at the single-cell level with current technologies is complicated by the lack of reliable methods to stably label particles over extended durations in vivo. Here we demonstrate that mass cytometry by time-of-flight provides a label-free approach for inorganic nanoparticle quantitation in cells. Furthermore, mass cytometry can enumerate AuNPs with a lower detection limit of ∼10 AuNPs (3 nm core size) in a single cell with tandem multiparameter cellular phenotyping. Using the cellular distribution insights, we selected an amphiphilic surface ligand-coated AuNP that targeted myeloid dendritic cells in lymph nodes as a peptide antigen carrier, substantially increasing the efficacy of a model vaccine in a B16-OVA melanoma mouse model. This technology provides a powerful new level of insight into nanoparticle fate in vivo.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Materials Science and Engineering
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1038/ncomms14069
