Loss of pRB and p107 disrupts cartilage development and promotes enchondroma formation
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Author(s)
Danielian, Paul S.
Lees, Jacqueline
Landman, A. S.
Date Issued
November 2012
Journal
Oncogene
Publisher
Nature Publishing Group
Citation
Landman, A S, P S Danielian, and J A Lees. “Loss of pRB and P107 Disrupts Cartilage Development and Promotes Enchondroma Formation.” Oncogene 32, no. 40 (November 12, 2012): 4798–4805.
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Author's final manuscript
Abstract
The pocket proteins pRB, p107 and p130 have established roles in regulating the cell cycle through the control of E2F activity. The pocket proteins regulate differentiation of a number of tissues in both cell cycle-dependent and -independent manners. Prior studies showed that mutation of p107 and p130 in the mouse leads to defects in cartilage development during endochondral ossification, the process by which long bones form. Despite evidence of a role for pRB in osteoblast differentiation, it is unknown whether it functions during cartilage development. Here, we show that mutation of Rb in the early mesenchyme of p107-mutant mice results in severe cartilage defects in the growth plates of long bones. This is attributable to inappropriate chondrocyte proliferation that persists after birth and leads to the formation of enchondromas in the growth plates as early as 8 weeks of age. Genetic crosses show that development of these tumorigenic lesions is E2f3 dependent. These results reveal an overlapping role for pRB and p107 in cartilage development, endochondral ossification and enchondroma formation that reflects their coordination of cell-cycle exit at appropriate developmental stages.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1038/onc.2012.496
