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Cytokine Profiling and Intra-Articular Injection of Autologous Platelet-Rich Plasma in Knee Osteoarthritis
Name
ijms-23-00890.pdf
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1.57 MB
Format
Adobe PDF
Checksum (MD5)
bd48dc524e320378c5d975bec2e09a12
Author(s)
Riewruja, Kanyakorn
Phakham, Suphattra
Sompolpong, Patlapa
Reantragoon, Rangsima
Tanavalee, Aree
Ngarmukos, Srihatach
Udomsinprasert, Wanvisa
Suantawee, Tanyawan
Dechsupa, Sinsuda
Honsawek, Sittisak
Date Issued
January 14, 2022
Publisher
Multidisciplinary Digital Publishing Institute
Citation
International Journal of Molecular Sciences 23 (2): 890 (2022)
Version
Final published version
Abstract
Osteoarthritis (OA) is a degenerative joint disease leading to joint pain and stiffness. Due to lack of effective treatments, physical and psychological disabilities caused by OA have a detrimental impact on the patient’s quality of life. Emerging evidence suggests that intra-articular injection of platelet-rich plasma (PRP) may provide favorable results since PRP comprises not only a high level of platelets but also a huge amount of cytokines, chemokines, and growth factors. However, the precise mechanism and standardization method remain uncertain. This study aimed to examine cytokine profiling in both PRP and platelet-poor plasma (PPP) of knee OA patients and to determine the effects of PRP on OA chondrocytes and knee OA patients. PRP contained a wide variety of cytokines, chemokines, growth factors, and autologous intra-articular PRP injection resulted in favorable outcomes in knee OA patients. Significant increases in levels of IL-1, IL-2, IL-7, IL-8, IL-9, IL-12, TNF-α, IL-17, PDGF-BB, bFGF, and MIP-1β were detected in PRP compared to PPP (p < 0.001). An in vitro study showed a marked increase in proliferation in OA chondrocytes cultured with PRP, compared to PPP and fetal bovine serum (p < 0.001). In a clinical study, knee OA patients treated with PRP showed improvement of physical function and pain, assessed by physical performance, Western Ontario and McMaster Universities Arthritis Index and visual analog scale. Our findings from both in vitro and clinical studies suggest that intra-articular PRP injection in knee OA patients may be a potential therapeutic strategy for alleviating knee pain and delaying the need for surgery.
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DOI of Published Version
http://dx.doi.org/10.3390/ijms23020890
