Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
Name
Sharei-2013-Cell squeezing as a.pdf
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336.83 KB
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Checksum (MD5)
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Author(s)
Sharei, Armon Reza
Cho, Nahyun
Mao, Shirley
Jackson, Emily L.
Poceviciute, Roberta
Zoldan, Janet
Langer, Robert
Jensen, Klavs F.
Adamo, Andrea, 1975-
Date Issued
November 2013
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corporation
Citation
Sharei, Armon, Nahyun Cho, Shirley Mao, Emily Jackson, Roberta Poceviciute, Andrea Adamo, Janet Zoldan, Robert Langer, and Klavs F Jensen. “Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform.” JoVE no. 81 (November 7, 2013).
Version
Final published version
Abstract
Rapid mechanical deformation of cells has emerged as a promising, vector-free method for intracellular delivery of macromolecules and nanomaterials. This technology has shown potential in addressing previously challenging applications; including, delivery to primary immune cells, cell reprogramming, carbon nanotube, and quantum dot delivery. This vector-free microfluidic platform relies on mechanical disruption of the cell membrane to facilitate cytosolic delivery of the target material. Herein, we describe the detailed method of use for these microfluidic devices including, device assembly, cell preparation, and system operation. This delivery approach requires a brief optimization of device type and operating conditions for previously unreported applications. The provided instructions are generalizable to most cell types and delivery materials as this system does not require specialized buffers or chemical modification/conjugation steps. This work also provides recommendations on how to improve device performance and trouble-shoot potential issues related to clogging, low delivery efficiencies, and cell viability.
MIT Department
Massachusetts Institute of Technology. Department of Chemical Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.3791/50980
