A Novel Imaging Approach for Early Detection of Prostate Cancer Based on Endogenous Zinc Sensing
Name
Ghosh et al Cancer Research.pdf
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6.55 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Ghosh, Subrata K.
Kim, Pilhan
Zhang, Xiao-an
Yu, Seok-Hyun
Moore, Anna V.
Lippard, Stephen J.
Medarova, Zdravka
Date Issued
July 2010
Journal
Cancer Research
Publisher
American Association for Cancer Research
Citation
Ghosh, Subrata K. et al. “A Novel Imaging Approach for Early Detection of Prostate Cancer Based on Endogenous Zinc Sensing.” Cancer Research 70.15 (2010) : 6119 -6127.
Version
Author's final manuscript
Abstract
The early detection of prostate cancer is a life-saving event in patients harboring potentially aggressive disease. With the development of malignancy, there is a dramatic reduction in the zinc content of prostate tissue associated with the inability of cancer cells to accumulate the ion. In the current study, we used endogenous zinc as an imaging biomarker for prostate cancer detection and progression monitoring. We employed a novel fluorescent sensor for mobile zinc (ZPP1) to detect and monitor the development of prostate cancer in a transgenic mouse model of prostate adenocarcinoma, using in vivo optical imaging correlated with biological fluid-based methods. We showed that the progression of prostate cancer could be monitored in vivo judging by the decreasing zinc content in the prostates of tumor-bearing mice in an age-dependent manner. In a novel quantitative assay, we determined the concentration of mobile zinc in both prostate cell lysates and mouse prostate extracts through simple titration of the ZPP1 sensor. Our findings fulfill the promise of zinc-based prostate cancer diagnostics with the prospect for immediate clinical translation.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-Noncommercial-Share Alike 3.0
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DOI of Published Version
https://doi.org/10.1158/0008-5472.CAN-10-1008
