LAB-1 Targets PP1 and Restricts Aurora B Kinase upon Entrance into Meiosis to Promote Sister Chromatid Cohesion
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Tzur-2012-LAB-1 Targets PP1 an.pdf
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Author(s)
Tzur, Yonatan B.
Carvalho, Carlos Egydio de
Nadarajan, Saravanapriah
Van Bostelen, Ivo
Gu, Yanjie
Chu, Diana S.
Colaiacovo, Monica P.
Cheeseman, Iain M
Date Issued
August 2012
Journal
PLoS Biology
Publisher
Public Library of Science
Citation
Tzur, Yonatan B. et al. “LAB-1 Targets PP1 and Restricts Aurora B Kinase Upon Entrance into Meiosis to Promote Sister Chromatid Cohesion.” Ed. R. Scott Hawley. PLoS Biology 10.8 (2012): e1001378.
Version
Final published version
Abstract
Successful execution of the meiotic program depends on the timely establishment and removal of sister chromatid cohesion. LAB-1 has been proposed to act in the latter by preventing the premature removal of the meiosis-specific cohesin REC-8 at metaphase I in C. elegans, yet the mechanism and scope of LAB-1 function remained unknown. Here we identify an unexpected earlier role for LAB-1 in promoting the establishment of sister chromatid cohesion in prophase I. LAB-1 and REC-8 are both required for the chromosomal association of the cohesin complex subunit SMC-3. Depletion of lab-1 results in partial loss of sister chromatid cohesion in rec-8 and coh-4 coh-3 mutants and further enhanced chromatid dissociation in worms where all three kleisins are mutated. Moreover, lab-1 depletion results in increased Aurora B kinase (AIR-2) signals in early prophase I nuclei, coupled with a parallel decrease in signals for the PP1 homolog, GSP-2. Finally, LAB-1 directly interacts with GSP-1 and GSP-2. We propose that LAB-1 targets the PP1 homologs to the chromatin at the onset of meiosis I, thereby antagonizing AIR-2 and cooperating with the cohesin complex to promote sister chromatid association and normal progression of the meiotic program.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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DOI of Published Version
https://doi.org/10.1371/journal.pbio.1001378
