Directing curli polymerization with DNA origami nucleators
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s41467-019-09369-6.pdf
Description
Published version
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1.84 MB
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Author(s)
Mao, Xiuhai
Li, Ke
Liu, Mengmeng
Wang, Xinyu
Zhao, Tianxin
An, Bolin
Cui, Mengkui
Li, Yingfeng
Pu, Jiahua
Li, Jiang
Date Issued
March 2019
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Mao, Xiuhai et al. "Directing curli polymerization with DNA origami nucleators." Nature Communications 10 (March 2019): 1395 © 2019 The Author(s)
Version
Final published version
Abstract
The physiological or pathological formation of fibrils often relies on molecular-scale nucleators that finely control the kinetics and structural features. However, mechanistic understanding of how protein nucleators mediate fibril formation in cells remains elusive. Here, we develop a CsgB-decorated DNA origami (CB-origami) to mimic protein nucleators in Escherichia coli biofilm that direct curli polymerization. We show that CB-origami directs curli subunit CsgA monomers to form oligomers and then accelerates fibril formation by increasing the proliferation rate of primary pathways. Fibrils grow either out from (departure mode) or towards the nucleators (arrival mode), implying two distinct roles of CsgB: as nucleation sites and as trap sites to capture growing nanofibrils in vicinity. Curli polymerization follows typical stop-and-go dynamics but exhibits a higher instantaneous elongation rate compared with independent fibril growth. This origami nucleator thus provides an in vitro platform for mechanistically probing molecular nucleation and controlling directional fibril polymerization for bionanotechnology.
MIT Department
Massachusetts Institute of Technology. Synthetic Biology Center
Massachusetts Institute of Technology. Research Laboratory of Electronics
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Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.1038/s41467-019-09369-6
