DNA Assembly in 3D Printed Fluidics
Name
Patrick-2015-DNA Assembly in 3D P.pdf
Size
1.28 MB
Format
Adobe PDF
Checksum (MD5)
a66b3e747b648568d8a1a49b8c267dff
Author(s)
Patrick, William G.
Levy, Taylor J.
Wang, Che-Wei
Rivera, Jaime J.
Voigt, Christopher A.
Oxman, Neri
Kong, David S.
Nielsen, Alec Andrew
Keating, Steven John
Mondragon-Palomino, Octavio
Date Issued
December 2015
Journal
PLOS ONE
Publisher
Public Library of Science
Citation
Patrick, William G., Alec A. K. Nielsen, Steven J. Keating, Taylor J. Levy, Che-Wei Wang, Jaime J. Rivera, Octavio Mondragon-Palomino, et al. “DNA Assembly in 3D Printed Fluidics.” Edited by Meni Wanunu. PLoS ONE 10, no. 12 (December 30, 2015): e0143636.
Version
Final published version
Abstract
The process of connecting genetic parts—DNA assembly—is a foundational technology for synthetic biology. Microfluidics present an attractive solution for minimizing use of costly reagents, enabling multiplexed reactions, and automating protocols by integrating multiple protocol steps. However, microfluidics fabrication and operation can be expensive and requires expertise, limiting access to the technology. With advances in commodity digital fabrication tools, it is now possible to directly print fluidic devices and supporting hardware. 3D printed micro- and millifluidic devices are inexpensive, easy to make and quick to produce. We demonstrate Golden Gate DNA assembly in 3D-printed fluidics with reaction volumes as small as 490 nL, channel widths as fine as 220 microns, and per unit part costs ranging from $0.61 to $5.71. A 3D-printed syringe pump with an accompanying programmable software interface was designed and fabricated to operate the devices. Quick turnaround and inexpensive materials allowed for rapid exploration of device parameters, demonstrating a manufacturing paradigm for designing and fabricating hardware for synthetic biology.
MIT Department
Lincoln Laboratory
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Media Laboratory
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0143636
