Single-cell measurement of plasmid copy number and promoter activity
Name
s41467-021-21734-y.pdf
Description
Published version
Size
1.87 MB
Format
Unknown
Checksum (MD5)
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Author(s)
Shao, Bin
Rammohan, Jayan
Anderson, Daniel A
Alperovich, Nina
Ross, David
Voigt, Christopher A
Date Issued
March 2021
Journal
Nature Communications
Publisher
Springer Science and Business Media LLC
Version
Final published version
Abstract
Accurate measurements of promoter activities are crucial for predictably building genetic systems. Here we report a method to simultaneously count plasmid DNA, RNA transcripts, and protein expression in single living bacteria. From these data, the activity of a promoter in units of RNAP/s can be inferred. This work facilitates the reporting of promoters in absolute units, the variability in their activity across a population, and their quantitative toll on cellular resources, all of which provide critical insights for cellular engineering.
MIT Department
Massachusetts Institute of Technology. Synthetic Biology Center
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1038/S41467-021-21734-Y
