Differentiation of normal and cancerous lung tissues by multiphoton imaging
Name
Wang-2009-Differentiation of n.pdf
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6.15 MB
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Checksum (MD5)
77669aefbfc2f55f0bf7d13f8a319474
Author(s)
Wang, Chun-Chin
Li, Feng-Chieh
Wu, Ruei-Jhih
Hovhannisyan, Vladimir
Lin, Wei-Chou
Lin, Sung-Jan
So, Peter T. C.
Dong, Chen-Yuan
Date Issued
August 2009
Journal
Journal of Biomedical Optics
Publisher
Society of Photo-Optical Instrumentation Engineers
Citation
Wang, Chun-Chin et al. “Differentiation of normal and cancerous lung tissues by multiphoton imaging.” Journal of Biomedical Optics 14.4 (2009): 044034-4. ©2009 Society of Photo-Optical Instrumentation Engineers
Version
Final published version
Abstract
We utilize multiphoton microscopy for the label-free diagnosis of noncancerous, lung adenocarcinoma (LAC), and lung squamous cell carcinoma (SCC) tissues from humans. Our results show that the combination of second-harmonic generation (SHG) and multiphoton excited autofluorescence (MAF) signals may be used to acquire morphological and quantitative information in discriminating cancerous from noncancerous lung tissues. Specifically, noncancerous lung tissues are largely fibrotic in structure, while cancerous specimens are composed primarily of tumor masses. Quantitative ratiometric analysis using MAF to SHG index (MAFSI) shows that the average MAFSI for noncancerous and LAC lung tissue pairs are 0.55±0.23 and 0.87±0.15, respectively. In comparison, the MAFSIs for the noncancerous and SCC tissue pairs are 0.50±0.12 and 0.72±0.13, respectively. Our study shows that nonlinear optical microscopy can assist in differentiating and diagnosing pulmonary cancer from noncancerous tissues.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
http://dx.doi.org/10.1117/1.3210768
