Molecular sled is an eleven-amino acid vehicle facilitating biochemical interactions via sliding components along DNA
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Mangel-2016-Molecular Sled.pdf
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Author(s)
Mangel, Walter F.
McGrath, William J.
Xiong, Kan
Graziano, Vito
Blainey, Paul C.
Date Issued
February 2016
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Mangel, Walter F., William J. McGrath, Kan Xiong, Vito Graziano, and Paul C. Blainey. “Molecular Sled Is an Eleven-Amino Acid Vehicle Facilitating Biochemical Interactions via Sliding Components Along DNA.” Nat Comms 7 (February 2, 2016): 10202.
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Final published version
Abstract
Recently, we showed the adenovirus proteinase interacts productively with its protein substrates in vitro and in vivo in nascent virus particles via one-dimensional diffusion along the viral DNA. The mechanism by which this occurs has heretofore been unknown. We show sliding of these proteins along DNA occurs on a new vehicle in molecular biology, a ‘molecular sled’ named pVIc. This 11-amino acid viral peptide binds to DNA independent of sequence. pVIc slides on DNA, exhibiting the fastest one-dimensional diffusion constant, 26±1.8 × 10[superscript 6] (bp)[superscript 2] s[superscript −1]. pVIc is a ‘molecular sled,’ because it can slide heterologous cargos along DNA, for example, a streptavidin tetramer. Similar peptides, for example, from the C terminus of β-actin or NLSIII of the p53 protein, slide along DNA. Characteristics of the ‘molecular sled’ in its milieu (virion, nucleus) have implications for how proteins in the nucleus of cells interact and imply a new form of biochemistry, one-dimensional biochemistry.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
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DOI of Published Version
https://doi.org/10.1038/ncomms10202
