Production of Clostridium difficile toxin in a medium totally free of both animal and dairy proteins or digests
Name
Fang-2009-Production of Clostr.pdf
Size
206.14 KB
Format
Adobe PDF
Checksum (MD5)
d7580b65a56d78e4f57f3c2b904973cc
Author(s)
Demain, Arnold L.
Gerson, Donald F.
Fang, Aiqi
Date Issued
July 2009
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publisher
United States National Academy of Sciences
Citation
Fang, Aiqi, Donald F Gerson, and Arnold L Demain. “Production of Clostridium difficile toxin in a medium totally free of both animal and dairy proteins or digests.” Proceedings of the National Academy of Sciences 106.32 (2009): 13225-13229. © 2009 National Academy of Sciences
Version
Final published version
Abstract
In the hope of developing a vaccine against Clostridium difficile based on its toxin(s), we have developed a fermentation medium for the bacterium that results in the formation of Toxin A and contains no meat or dairy products, thus obviating the problem of possible prion diseases. Particular preparations of hydrolyzed soy proteins, especially Soy Peptone A3, have been found to replace both the meat/dairy product tryptone in the preparation of working cell banks and seed media, and NZ-Soy BL4 does the same in the fermentation medium. These replacements yield even higher toxin titers.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
Persistent DSpace Link
DOI of Published Version
http://dx.doi.org/10.1073/pnas.0906425106
