Measurement of bacterial promoter absolute activity and determination of dynamic performance in cell-free expression system by qPCR
Name
BBFRFC58.pdf
Size
88.1 KB
Format
Adobe PDF
Checksum (MD5)
52843037b9d7b79f908ee8adb0131b14
Author(s)
Olchowik, Anna
Lower, Michal
Moreno, Cherry
Pankowski, Jaroslaw
Date Issued
December 5, 2010
Series/Report no.
BBF RFC;58
Abstract
The purpose of this RFC is to provide standard methodology for the measurement of the absolute strength of bacterial promoters. The absolute activity can be experimentally measured in Moles of RNA per Moles of DNA in time, and therefore it is can be expressed in PoPS [1]
Subjects
measurement
Terms of Use
Attribution-No Derivative Works 3.0 United States
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