Design and Fabrication of Ultralight Weight, Adjustable Multi-electrode Probes for Electrophysiological Recordings in Mice
Name
Brunetti-2014-Design and Fabricati.pdf
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570.81 KB
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Checksum (MD5)
51952ac54986f8b97a667c681a59b180
Author(s)
Brunetti, Philip M.
Wimmer, Ralf D.
Liang, Li
Siegle, Joshua H.
Voigts, Jakob
Halassa, Michael M.
Wilson, Matthew A.
Date Issued
September 2014
Journal
Journal of Visualized Experiments
Publisher
MyJoVE Corporation
Citation
Brunetti, Philip M., Ralf D. Wimmer, Li Liang, Joshua H. Siegle, Jakob Voigts, Matthew Wilson, and Michael M. Halassa. “Design and Fabrication of Ultralight Weight, Adjustable Multi-Electrode Probes for Electrophysiological Recordings in Mice.” JoVE no. 91 (2014).
Version
Final published version
Abstract
The number of physiological investigations in the mouse, mus musculus, has experienced a recent surge, paralleling the growth in methods of genetic targeting for microcircuit dissection and disease modeling. The introduction of optogenetics, for example, has allowed for bidirectional manipulation of genetically-identified neurons, at an unprecedented temporal resolution. To capitalize on these tools and gain insight into dynamic interactions among brain microcircuits, it is essential that one has the ability to record from ensembles of neurons deep within the brain of this small rodent, in both head-fixed and freely behaving preparations. To record from deep structures and distinct cell layers requires a preparation that allows precise advancement of electrodes towards desired brain regions. To record neural ensembles, it is necessary that each electrode be independently movable, allowing the experimenter to resolve individual cells while leaving neighboring electrodes undisturbed. To do both in a freely behaving mouse requires an electrode drive that is lightweight, resilient, and highly customizable for targeting specific brain structures.
A technique for designing and fabricating miniature, ultralight weight, microdrive electrode arrays that are individually customizable and easily assembled from commercially available parts is presented. These devices are easily scalable and can be customized to the structure being targeted; it has been used successfully to record from thalamic and cortical regions in a freely behaving animal during natural behavior.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
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Creative Commons Attribution
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DOI of Published Version
https://doi.org/10.3791/51675
