Contact-Dependent Killing by Caulobacter Crescentus via Cell Surface-Associated, Glycine Zipper Proteins
Name
Garcia-Bayona-2017-Contact-dependent killing b.pdf
Size
2.03 MB
Format
Adobe PDF
Checksum (MD5)
16297884d3f277a02e3be8fc82b495c0
Author(s)
Garcia Bayona, Leonor
Guo, Monica S
Laub, Michael T
Date Issued
March 2017
Journal
eLife
Publisher
eLife Sciences Publications, Ltd.
Citation
García-Bayona, Leonor; Guo, Monica S and Laub, Michael T. “Contact-Dependent Killing by Caulobacter Crescentus via Cell Surface-Associated, Glycine Zipper Proteins.” eLife 6 (March 2017): e24869 © 2017, García-Bayona et al
Version
Final published version
Abstract
Most bacteria are in fierce competition with other species for limited nutrients. Some bacteria can kill nearby cells by secreting bacteriocins, a diverse group of proteinaceous antimicrobials. However, bacteriocins are typically freely diffusible, and so of little value to planktonic cells in aqueous environments. Here, we identify an atypical two-protein bacteriocin in the α-proteobacterium Caulobacter crescentus that is retained on the surface of producer cells where it mediates cell contact-dependent killing. The bacteriocin-like proteins CdzC and CdzD harbor glycine-zipper motifs, often found in amyloids, and CdzC forms large, insoluble aggregates on the surface of producer cells. These aggregates can drive contact-dependent killing of other organisms, or Caulobacter cells not producing the CdzI immunity protein. The Cdz system uses a type I secretion system and is unrelated to previously described contact-dependent inhibition systems. However, Cdz-like systems are found in many bacteria, suggesting that this form of contact-dependent inhibition is common.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution 4.0 International License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.7554/eLife.24869
