Reflection phase microscopy using spatio-temporal coherence of light
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optica-5-11-1468.pdf
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Author(s)
Choi, Youngwoon
Hosseini, Poorya
Kang, Jeon Woong
Kang, Sungsam
Yang, Taeseok Daniel
Hyeon, Min Gyu
Kim, Beop-Min
So, Peter T. C.
Yaqoob, Zahid
Date Issued
November 2018
Journal
Optica
Publisher
Oxford University Press
Citation
Choi, Youngwoon, Poorya Hosseini, Jeon Woong Kang, Sungsam Kang, Taeseok Daniel Yang, Min Gyu Hyeon, Beop-Min Kim, Peter T. C. So, and Zahid Yaqoob. “Reflection Phase Microscopy Using Spatio-Temporal Coherence of Light.” Optica 5, no. 11 (November 15, 2018): 1468.
Version
Final published version
Abstract
Many disease states are associated with cellular biomechanical changes as markers. Label-free phase microscopes are used to quantify thermally driven interface fluctuations, which allow the deduction of important cellular rheological properties. Here, the spatio-temporal coherence of light was used to implement a high-speed reflection phase microscope with superior depth selectivity and higher phase sensitivity. Nanometric scale motion of cytoplasmic structures can be visualized with fine details and three-dimensional resolution. Specifically, the spontaneous fluctuation occurring on the nuclear membrane of a living cell was observed at video rate. By converting the reflection phase into displacement, the sensitivity in quantifying nuclear membrane fluctuation was found to be about one nanometer. A reflection phase microscope can potentially elucidate biomechanical mechanisms of pathological and physiological processes.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Chemistry
Massachusetts Institute of Technology. Department of Mechanical Engineering
Massachusetts Institute of Technology. Spectroscopy Laboratory
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1364/OPTICA.5.001468
