Experimental design and analysis for high-parameter spatial omics
Name
baker-eagb-phd-biology-2022-thesis .pdf
Description
Thesis PDF
Size
73.81 MB
Format
Adobe PDF
Checksum (MD5)
4887f55e33c4dae8b6c6c5b905b9b80a
Author(s)
Baker, Ethan Alexander García
Advisor(s)
Regev, Aviv
Date Issued
May 2022
Publisher
Massachusetts Institute of Technology
Abstract
Recent decades have witnessed the dawn of an era of molecular biology as a data science. Technological advancements have enabled high-parameter molecular measurements (“omics”) to progress from bulk data, averaged across entire tissues or organisms, to single-cell measurements, which enable exploration of the vast diversity between individual cells, to a new frontier, spatial omics, which enables rich molecular measurements of single cells in their native tissue context. Spatial omics methods complement the rich history of histopathology to unlock new avenues to explore the spatial components of tissue biology. However, high-parameter spatial omics data present unique statistical and computational challenges, and substantial work is required to ensure that findings of spatial omics experiments are actionable in the laboratory and clinic. Here, I examine the underlying statistical properties of spatial biology to propose a general framework for experimental design in spatial omics, introduce an approximate generative model of tissue structure, and demonstrate methods for semantic segmentation and community detection in spatial omics. Finally, I share an outlook on how, when considered jointly, these contributions represent first steps towards optimal experimental design for spatial omics.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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