Chromatin regulation at the frontier of synthetic biology
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Collins_Chromatin regulation.pdf
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1.02 MB
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Author(s)
Joung, J. Keith
Khalil, Ahmad S.
Keung, Albert Jun Qi
Collins, James J.
Date Issued
February 2015
Journal
Nature Reviews Genetics
Publisher
Nature Publishing Group
Citation
Keung, Albert J. et al. “Chromatin Regulation at the Frontier of Synthetic Biology.” Nature Reviews Genetics 16.3 (2015): 159–171.
Version
Author's final manuscript
Abstract
As synthetic biology approaches are extended to diverse applications throughout medicine, biotechnology and basic biological research, there is an increasing need to engineer yeast, plant and mammalian cells. Eukaryotic genomes are regulated by the diverse biochemical and biophysical states of chromatin, which brings distinct challenges, as well as opportunities, over applications in bacteria. Recent synthetic approaches, including 'epigenome editing', have allowed the direct and functional dissection of many aspects of physiological chromatin regulation. These studies lay the foundation for biomedical and biotechnological engineering applications that could take advantage of the unique combinatorial and spatiotemporal layers of chromatin regulation to create synthetic systems of unprecedented sophistication.
MIT Department
Institute for Medical Engineering and Science
Massachusetts Institute of Technology. Department of Biological Engineering
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1038/nrg3900
