Principles of designing interpretable optogenetic behavior experiments
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Boyden_Principles of.pdf
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238.05 KB
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Author(s)
Boyden, Edward Stuart
Allen, Brian Douglas
Singer, Annabelle
Date Issued
March 2015
Journal
Learning & Memory
Publisher
Cold Spring Harbor Laboratory Press
Citation
Allen, Brian D., Annabelle C. Singer, and Edward S. Boyden. “Principles of Designing Interpretable Optogenetic Behavior Experiments.” Learning & Memory 22, no. 4 (March 18, 2015): 232–238.
Version
Final published version
Abstract
Over the last decade, there has been much excitement about the use of optogenetic tools to test whether specific cells, regions, and projection pathways are necessary or sufficient for initiating, sustaining, or altering behavior. However, the use of such tools can result in side effects that can complicate experimental design or interpretation. The presence of optogenetic proteins in cells, the effects of heat and light, and the activity of specific ions conducted by optogenetic proteins can result in cellular side effects. At the network level, activation or silencing of defined neural populations can alter the physiology of local or distant circuits, sometimes in undesired ways. We discuss how, in order to design interpretable behavioral experiments using optogenetics, one can understand, and control for, these potential confounds.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1101/lm.038026.114
