Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition)
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kaup-12-01-1100356.pdf
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8.91 MB
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Author(s) • •
Amon, Angelika B.
Santaguida, Stefano
Yang, Peixin
Date Issued
January 2016
Journal
Autophagy
Publisher
Informa UK Limited
Citation
Klionsky, Daniel J., et al. “Guidelines for the Use and Interpretation of Assays for Monitoring Autophagy (3rd Edition).” Autophagy, vol. 12, no. 1, Jan. 2016, pp. 1–222.
Version
Author's final manuscript
Abstract
In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different
organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1080/15548627.2015.1100356