Noninvasive monitoring of single-cell mechanics by acoustic scattering
Name
emss-81182.pdf
Description
Accepted version
Size
1.51 MB
Format
Adobe PDF
Checksum (MD5)
e3ecf7253765da3323a0ff88dac81b48
Author(s)
Kang, Joon Ho
Miettinen, Teemu P
Chen, Lynna
Olcum, Selim A.
Katsikis, Georgios
Doyle, Patrick S
Manalis, Scott R
Date Issued
February 2019
Journal
Nature Methods
Publisher
Springer Science and Business Media LLC
Citation
Kang, Joon Ho et al. "Noninvasive monitoring of single-cell mechanics by acoustic scattering." Nature Methods 16, 3 (February 2019): 263–269 © 2019 The Author(s)
Version
Author's final manuscript
Abstract
The monitoring of mechanics in a single cell throughout the cell cycle has been hampered by the invasiveness of mechanical measurements. Here we quantify mechanical properties via acoustic scattering of waves from a cell inside a fluid-filled vibrating cantilever with a temporal resolution of < 1 min. Through simulations, experiments with hydrogels and the use of chemically perturbed cells, we show that our readout, the size-normalized acoustic scattering (SNACS), measures stiffness. To demonstrate the noninvasiveness of SNACS over successive cell cycles, we used measurements that resulted in deformations of < 15 nm. The cells maintained constant SNACS throughout interphase but showed dynamic changes during mitosis. Our work provides a basis for understanding how growing cells maintain mechanical integrity, and demonstrates that acoustic scattering can be used to noninvasively probe subtle and transient dynamics.
MIT Department
Massachusetts Institute of Technology. Department of Physics
Massachusetts Institute of Technology. Department of Chemical Engineering
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Mechanical Engineering
Koch Institute for Integrative Cancer Research at MIT
Terms of Use
Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/s41592-019-0326-x
