High-throughput hyperdimensional vertebrate phenotyping
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Yanik_High-throughput.pdf
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Author(s)
Pardo-Martin, Carlos
Allalou, Amin Mohamed
Medina, Jaime
Eimon, Peter
Wählby, Carolina
Yanik, Mehmet Fatih
Date Issued
February 2013
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Pardo-Martin, Carlos, Amin Allalou, Jaime Medina, Peter M. Eimon, Carolina Wählby, and Mehmet Fatih Yanik. “High-Throughput Hyperdimensional Vertebrate Phenotyping.” Nature Communications 4 (February 12, 2013): 1467.
Version
Author's final manuscript
Abstract
Most gene mutations and biologically active molecules cause complex responses in animals that cannot be predicted by cell culture models. Yet animal studies remain too slow and their analyses are often limited to only a few readouts. Here we demonstrate high-throughput optical projection tomography with micrometre resolution and hyperdimensional screening of entire vertebrates in tens of seconds using a simple fluidic system. Hundreds of independent morphological features and complex phenotypes are automatically captured in three dimensions with unprecedented speed and detail in semitransparent zebrafish larvae. By clustering quantitative phenotypic signatures, we can detect and classify even subtle alterations in many biological processes simultaneously. We term our approach hyperdimensional in vivo phenotyping. To illustrate the power of hyperdimensional in vivo phenotyping, we have analysed the effects of several classes of teratogens on cartilage formation using 200 independent morphological measurements, and identified similarities and differences that correlate well with their known mechanisms of actions in mammals.
MIT Department
Harvard University--MIT Division of Health Sciences and Technology
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Electrical Engineering and Computer Science
Massachusetts Institute of Technology. Research Laboratory of Electronics
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/ncomms2475
