Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains
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Author(s)
Cong, Le
Zhou, Ruhong
Kuo, Yu-chi
Cunniff, Margaret Mary
Zhang, Feng
Date Issued
July 2012
Journal
Nature Communications
Publisher
Nature Publishing Group
Citation
Cong, Le, Ruhong Zhou, Yu-chi Kuo, Margaret Cunniff, and Feng Zhang. "Comprehensive interrogation of natural TALE DNA-binding modules and transcriptional repressor domains." Nature Communications 3:968 (2012) p.1-6.
Version
Author's final manuscript
Abstract
Transcription activator-like effectors are sequence-specific DNA-binding proteins that harbour modular, repetitive DNA-binding domains. Transcription activator-like effectors have enabled the creation of customizable designer transcriptional factors and sequence-specific nucleases for genome engineering. Here we report two improvements of the transcription activator-like effector toolbox for achieving efficient activation and repression of endogenous gene expression in mammalian cells. We show that the naturally occurring repeat-variable diresidue Asn-His (NH) has high biological activity and specificity for guanine, a highly prevalent base in mammalian genomes. We also report an effective transcription activator-like effector transcriptional repressor architecture for targeted inhibition of transcription in mammalian cells. These findings will improve the precision and effectiveness of genome engineering that can be achieved using transcription activator-like effectors.
MIT Department
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
McGovern Institute for Brain Research at MIT
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1038/ncomms1962
