Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors
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Author(s)
Villani, Alexandra-Chloé
Satija, Rahul
Reynolds, Gary
Sarkizova, Siranush
Shekhar, Karthik
Fletcher, James
Griesbeck, Morgane
Butler, Andrew
Zheng, Shiwei
Lazo, Suzan
Date Issued
April 2017
Journal
Science
Publisher
American Association for the Advancement of Science (AAAS)
Citation
Villani, Alexandra-Chloé et al. “Single-Cell RNA-Seq Reveals New Types of Human Blood Dendritic Cells, Monocytes, and Progenitors.” Science 356, 6335 (April 2017): eaah4573 © 2017 American Association for the Advancement of Science
Version
Author's final manuscript
Abstract
Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C+ subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1126/SCIENCE.AAH4573
