A System to Study Aneuploidy In Vivo
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nihms750493.pdf
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Author(s)
Pfau, Sarah Jeanne
Amon, Angelika B
Date Issued
March 2016
Journal
Cold Spring Harbor Symposia on Quantitative Biology
Publisher
Cold Spring Harbor Laboratory
Citation
Pfau, Sarah J., and Angelika Amon. “A System to Study Aneuploidy In Vivo.” Cold Spring Harbor Symposia on Quantitative Biology, vol. 80, 2016, pp. 93–101.
Version
Author's final manuscript
Abstract
Aneuploidy, an imbalanced chromosome number, is associated with both cancer and developmental disorders such as Down syndrome (DS). To determine how aneuploidy affects cellular and organismal physiology, we have developed a system to evaluate aneuploid cell fitness in vivo. By transplanting hematopoietic stem cells (HSCs) into recipient mice after ablation of recipient hematopoiesis by lethal irradiation, we can directly compare the fitness of HSCs derived from a range of aneuploid mouse models with that of euploid HSCs. This experimental system can also be adapted to assess the interplay between aneuploidy and tumorigenesis. We hope that further characterization of aneuploid cells in vivo will provide insight both into the origins of hematopoietic phenotypes observed in DS individuals as well as the role of different types of aneuploid cells in the genesis of cancers of the blood.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Koch Institute for Integrative Cancer Research at MIT
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Creative Commons Attribution-Noncommercial-Share Alike
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DOI of Published Version
https://doi.org/10.1101/SQB.2015.80.027193
