A highly conserved protein of unknown function in Sinorhizobium meliloti affects sRNA regulation similar to Hfq
Name
Panedy-2011-A highly conserved protein of unknown function in.pdf
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Author(s)
Pandey, Shree P.
Minesinger, Brenda
Kumar, Janesh
Walker, Graham C.
Date Issued
February 2011
Journal
Nucleic Acids Research
Publisher
Oxford University Press
Citation
Pandey, S. P. et al. “A Highly Conserved Protein of Unknown Function in Sinorhizobium Meliloti Affects sRNA Regulation Similar to Hfq.” Nucleic Acids Research 39.11 (2011): 4691–4708. Web.
Version
Final published version
Abstract
The SMc01113/YbeY protein, belonging to the UPF0054 family, is highly conserved in nearly every bacterium. However, the function of these proteins still remains elusive. Our results show that SMc01113/YbeY proteins share structural similarities with the MID domain of the Argonaute (AGO) proteins, and might similarly bind to a small-RNA (sRNA) seed, making a special interaction with the phosphate on the 5′-side of the seed, suggesting they may form a component of the bacterial sRNA pathway. Indeed, eliminating SMc01113/YbeY expression in Sinorhizobium meliloti produces symbiotic and physiological phenotypes strikingly similar to those of the hfq mutant. Hfq, an RNA chaperone, is central to bacterial sRNA-pathway. We evaluated the expression of 13 target genes in the smc01113 and hfq mutants. Further, we predicted the sRNAs that may potentially target these genes, and evaluated the accumulation of nine sRNAs in WT and smc01113 and hfq mutants. Similar to hfq, smc01113 regulates the accumulation of sRNAs as well as the target mRNAs. AGOs are central components of the eukaryotic sRNA machinery and conceptual parallels between the prokaryotic and eukaryotic sRNA pathways have long been drawn. Our study provides the first line of evidence for such conceptual parallels. Furthermore, our investigation gives insights into the sRNA-mediated regulation of stress adaptation in S. meliloti.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Creative Commons Attribution Non-Commercial
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DOI of Published Version
https://doi.org/10.1093/nar/gkr060
