Nutritional Control of Elongation of DNA Replication by (p)ppGpp
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Grossman_Nutritional control.pdf
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685.85 KB
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Author(s)
Wang, Jue D.
Sanders, Glenn M.
Grossman, Alan Davis
Date Issued
March 2007
Journal
Cell
Publisher
Elsevier
Citation
Wang, Jue D., Glenn M. Sanders, and Alan D. Grossman. “Nutritional Control of Elongation of DNA Replication by (p)ppGpp.” Cell 128, no. 5 (March 2007): 865-875. Copyright © 2007 Elsevier Inc.
Version
Final published version
Abstract
DNA replication is highly regulated in most organisms. Although much research has focused on mechanisms that regulate initiation of replication, mechanisms that regulate elongation of replication are less well understood. We characterized a mechanism that regulates replication elongation in the bacterium Bacillus subtilis. Replication elongation was inhibited within minutes after amino acid starvation, regardless of where the replication forks were located on the chromosome. We found that small nucleotides ppGpp and pppGpp, which are induced upon starvation, appeared to inhibit replication directly by inhibiting primase, an essential component of the replication machinery. The replication forks arrested with (p)ppGpp did not recruit the recombination protein RecA, indicating that the forks are not disrupted. (p)ppGpp appear to be part of a surveillance mechanism that links nutrient availability to replication by rapidly inhibiting replication in starved cells, thereby preventing replication-fork disruption. This control may be important for cells to maintain genomic integrity.
MIT Department
Massachusetts Institute of Technology. Department of Biology
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Article is made available in accordance with the publisher's policy and may be subject to US copyright law. Please refer to the publisher's site for terms of use.
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DOI of Published Version
https://doi.org/10.1016/j.cell.2006.12.043
