Sub-millisecond optogenetic control of neuronal firing with two-photon holographic photoactivation of Chronos
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Author(s)
Ronzitti, E.
Conti, R.
Zampini, V.
Tanese, D.
Foust, A.J.
Papagiakoumou, E.
Emiliani, V.
Klapoetke, Nathan Cao
Boyden, Edward
Date Issued
November 2017
Journal
The Journal of Neuroscience
Publisher
Society for Neuroscience
Citation
Ronzitti, E. et al. “Sub-Millisecond Optogenetic Control of Neuronal Firing with Two-Photon Holographic Photoactivation of Chronos.” The Journal of Neuroscience 37, 44 (October 2017): 10679-10689 © 2017 The Authors
Version
Final published version
Abstract
Optogenetic neuronal network manipulation promises to unravel a long-standing mystery in neuroscience: how does microcircuit activity relate causally to behavioral and pathological states? The challenge to evoke spikes with high spatial and temporal complexity necessitates further joint development of light-delivery approaches and custom opsins. Two-photon (2P) light-targeting strategies demonstrated in-depth generation of action potentials in photosensitive neurons both in vitro and in vivo, but thus far lack the temporal precision necessary to induce precisely timed spiking events. Here, we show that efficient current integration enabled by 2P holographic amplified laser illumination of Chronos, a highly light-sensitive and fast opsin, can evoke spikes with submillisecond precision and repeated firing up to 100 Hz in brain slices from Swiss male mice. These results pave the way for optogenetic manipulation with the spatial and temporal sophistication necessary to mimic natural microcircuit activity.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Massachusetts Institute of Technology. Department of Brain and Cognitive Sciences
Massachusetts Institute of Technology. Media Laboratory
McGovern Institute for Brain Research at MIT
Terms of Use
Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1523/JNEUROSCI.1246-17.2017
