Automated electrochemical oxygen sensing using a 3D-printed microfluidic lab-on-a-chip system
Name
d4lc00962b.pdf
Description
Published version
Size
3.44 MB
Format
Adobe PDF
Checksum (MD5)
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Author(s)
Kaufman, Daniel
Winkler, Steffen
Heuer, Christopher
Shibli, Ahed
Snezhko, Alexander
Livshits, Gideon I
Bahnemann, Janina
Ben-Yoav, Hadar
Date Issued
December 28, 2024
Journal
Lab on a Chip
Publisher
Royal Society of Chemistry
Citation
Kaufman, Daniel, Winkler, Steffen, Heuer, Christopher, Shibli, Ahed, Snezhko, Alexander et al. 2024. "Automated electrochemical oxygen sensing using a 3D-printed microfluidic lab-on-a-chip system." Lab on a Chip, 25 (6).
Version
Final published version
Abstract
Dissolved oxygen is crucial for metabolism, growth, and other complex physiological and pathological processes; however, standard physiological models (such as organ-on-chip systems) often use ambient oxygen levels, which do not reflect the lower levels that are typically found in vivo. Additionally, the local generation of reactive oxygen species (ROS; a key factor in physiological systems) is often overlooked in biology-mimicking models. Here, we present a microfluidic system that integrates electrochemical dissolved oxygen sensors with lab-on-a-chip technology to monitor the physiological oxygen concentrations and generate hydrogen peroxide (H2O2; a specific ROS). This microfluidic lab-on-a-chip system was fabricated using high-resolution 3D printing technology in a one-step process. It incorporates a micromixer, an on-chip bubble-trap, an electrochemical cell with fabricated gold or platinum black-coated working electrodes as well as an Ag/AgCl reference electrode, and a commercial optical oxygen sensor for validation. This device enables an automated variation of the oxygen levels as well as sensitive electrochemical oxygen monitoring (limit of detection = 11.9 ± 0.3 μM), with a statistically significant correlation with the optical sensor. The proposed system can serve as a tool to characterize and evaluate custom-made electrodes. Indeed, we envision that in the future it will be used to regulate dissolved oxygen levels and oxygen species in real time in organ-on-chip systems.
MIT Department
Massachusetts Institute of Technology. Office of Strategic Alliances and Technology Transfer. Corporate Relations
Terms of Use
Creative Commons Attribution-Noncommercial
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DOI of Published Version
https://doi.org/10.1039/D4LC00962B
