Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants
Name
advs.201903551.pdf
Size
2.6 MB
Format
Adobe PDF
Checksum (MD5)
8a5041225aa53c81c5b62c002b2ea044
Author(s)
Cao, Yunteng
Lim, Eugene J.
Xu, Menglong
Weng, Jing-Ke
Marelli, Benedetto
Date Issued
April 22, 2020
Journal
Advanced Science
Publisher
Wiley
Citation
Cao, Yunteng et al. "Precision Delivery of Multiscale Payloads to Tissue‐Specific Targets in Plants." Advanced Science (April 2020): 1903551 © 2020 The Authors.
Version
Final published version
Abstract
The precise deployment of functional payloads to plant tissues is a new approach to help advance the fundamental understanding of plant biology and accelerate plant engineering. Here, the design of a silk‐based biomaterial is reported to fabricate a microneedle‐like device, dubbed “phytoinjector,” capable of delivering a variety of payloads ranging from small molecules to large proteins into specific loci of various plant tissues. It is shown that phytoinjector can be used to deliver payloads into plant vasculature to study material transport in xylem and phloem and to perform complex biochemical reactions in situ. In another application, it is demonstrated Agrobacterium ‐mediated gene transfer to shoot apical meristem (SAM) and leaves at various stages of growth. Tuning of the material composition enables the fabrication of another device, dubbed “phytosampler,” which is used to precisely sample plant sap. The design of plant‐specific biomaterials to fabricate devices for drug delivery in planta opens new avenues to enhance plant resistance to biotic and abiotic stresses, provides new tools for diagnostics, and enables new opportunities in plant engineering.
Subjects
General Engineering
General Physics and Astronomy
General Materials Science
Medicine (miscellaneous)
General Chemical Engineering
Biochemistry, Genetics and Molecular Biology (miscellaneous)
MIT Department
Massachusetts Institute of Technology. Department of Civil and Environmental Engineering
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
Terms of Use
Creative Commons Attribution 4.0 International license
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1002/advs.201903551
