A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue
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Lippard_A far-red.pdf
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Author(s)
Khan, Mustafa
Georgiou, John
Luyben, Thomas T.
Roder, John C.
Okamoto, Kenichi
Rivera Fuentes, Pablo Marcelo
Wrobel, Alexandra T.
Zastrow, Melissa L.
Lippard, Stephen J.
Date Issued
January 2015
Journal
Chemical Science
Publisher
Royal Society of Chemistry, The
Citation
Rivera-Fuentes, Pablo; Wrobel, Alexandra T.; Zastrow, Melissa L.; Khan, Mustafa; Georgiou, John; Luyben, Thomas T.; Roder, John C.; Okamoto, Kenichi and Lippard, Stephen J. "A far-red emitting probe for unambiguous detection of mobile zinc in acidic vesicles and deep tissue." Chemical Science 2015, 6 (January 2015): 1944-1948 © 2015 The Royal Society of Chemistry
Version
Final published version
Abstract
Imaging mobile zinc in acidic environments remains challenging because most small-molecule optical probes display pH-dependent fluorescence. Here we report a reaction-based sensor that detects mobile zinc unambiguously at low pH. The sensor responds reversibly and with a large dynamic range to exogenously applied Zn²⁺ in lysosomes of HeLa cells, endogenous Zn²⁺ in insulin granules of MIN6 cells, and zinc-rich mossy fiber boutons in hippocampal tissue from mice. This long-wavelength probe is compatible with the green-fluorescent protein, enabling multicolor imaging, and facilitates visualization of mossy fiber boutons at depths of >100 μm, as demonstrated by studies in live tissue employing two-photon microscopy.
MIT Department
Massachusetts Institute of Technology. Department of Chemistry
Terms of Use
Creative Commons Attribution-NonCommercial 3.0 Unported licence
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DOI of Published Version
https://doi.org/10.1039/c4sc03388d
