The rate of protein synthesis in hematopoietic stem cells is limited partly by 4E-BPs
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Signer_The rate of protein.pdf
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Author(s)
Signer, Robert A.J.
Qi, Le
Zhao, Zhiyu
Thompson, David
Sigova, Alla A.
Fan, Zi Peng
DeMartino, George N.
Sonenberg, Nahum
Morrison, Sean J.
Young, Richard A.
Date Issued
October 2016
Journal
Genes & Development
Publisher
Cold Spring Harbor Laboratory Press
Citation
Signer, Robert A.J. et al. “The Rate of Protein Synthesis in Hematopoietic Stem Cells Is Limited Partly by 4E-BPs.” Genes & Development 30.15 (2016): 1698–1703.
Version
Final published version
Abstract
Adult stem cells must limit their rate of protein synthesis, but the underlying mechanisms remain largely unexplored. Differences in protein synthesis among hematopoietic stem cells (HSCs) and progenitor cells did not correlate with differences in proteasome activity, total RNA content, mRNA content, or cell division rate. However, adult HSCs had more hypophosphorylated eukaryotic initiation factor 4E-binding protein 1 (4E-BP1) and 4E-BP2 as compared with most other hematopoietic progenitors. Deficiency for 4E-BP1 and 4E-BP2 significantly increased global protein synthesis in HSCs, but not in other hematopoietic progenitors, and impaired their reconstituting activity, identifying a mechanism that promotes HSC maintenance by attenuating protein synthesis.
MIT Department
Massachusetts Institute of Technology. Department of Biology
Whitehead Institute for Biomedical Research
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Creative Commons Attribution 4.0 International License
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DOI of Published Version
https://doi.org/10.1101/gad.282756.116
