Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice
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Sakaguchi-2015-Inhibiting the Activ.pdf
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Author(s)
Sakaguchi, Masanori
Kim, Karam
Yu, Lily Mae Yee
Hashikawa, Yoshiko
Sekine, Yukiko
Okumura, Yuki
Kawano, Masako
Hayashi, Masanobu
Kumar, Deependra
McHugh, Thomas J.
Date Issued
June 2015
Journal
PLOS ONE
Publisher
Public Library of Science
Citation
Sakaguchi, Masanori, Karam Kim, Lily Mae Yee Yu, Yoshiko Hashikawa, Yukiko Sekine, Yuki Okumura, Masako Kawano, et al. “Inhibiting the Activity of CA1 Hippocampal Neurons Prevents the Recall of Contextual Fear Memory in Inducible ArchT Transgenic Mice.” Edited by Masuo Ohno. PLoS ONE 10, no. 6 (June 15, 2015): e0130163.
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Final published version
Abstract
The optogenetic manipulation of light-activated ion-channels/pumps (i.e., opsins) can reversibly activate or suppress neuronal activity with precise temporal control. Therefore, optogenetic techniques hold great potential to establish causal relationships between specific neuronal circuits and their function in freely moving animals. Due to the critical role of the hippocampal CA1 region in memory function, we explored the possibility of targeting an inhibitory opsin, ArchT, to CA1 pyramidal neurons in mice. We established a transgenic mouse line in which tetracycline trans-activator induces ArchT expression. By crossing this line with a CaMKIIα-tTA transgenic line, the delivery of light via an implanted optrode inhibits the activity of excitatory CA1 neurons. We found that light delivery to the hippocampus inhibited the recall of a contextual fear memory. Our results demonstrate that this optogenetic mouse line can be used to investigate the neuronal circuits underlying behavior.
MIT Department
Massachusetts Institute of Technology. Media Laboratory
Program in Media Arts and Sciences (Massachusetts Institute of Technology)
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DOI of Published Version
https://doi.org/10.1371/journal.pone.0130163
