Assisted assembly: how to improve a de novo genome assembly by using related species
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gb-2009-10-8-r88.pdf
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173.58 KB
Format
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Checksum (MD5)
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Author(s)
Gnerre, Sante
Lindblad-Toh, Kerstin
Jaffe, David B.
Lander, Eric Steven
Date Issued
August 2009
Journal
Genome Biology
Publisher
BioMed Central Ltd
Citation
Gnerre, Sante et al. “Assisted Assembly: How to Improve a De Novo Genome Assembly by Using Related Species.” Genome Biology 10.8 (2009): R88. Web. 24 Feb. 2012.
Version
Final published version
Abstract
We describe a new assembly algorithm, where a genome assembly with low sequence coverage, either throughout the genome or locally, due to cloning bias, is considerably improved through an assisting process via a related genome. We show that the information provided by aligning the whole-genome shotgun reads of the target against a reference genome can be used to substantially improve the quality of the resulting assembly.
MIT Department
Broad Institute of MIT and Harvard
Massachusetts Institute of Technology. Department of Biology
Terms of Use
Creative Commons Attribution
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1186/gb-2009-10-8-r88
