6 Principles and applications of temporal-focusing wide-field two-photon microscopy
Name
[9783110429985 - Multiphoton Microscopy and Fluorescence Lifetime Imaging] 6 Principles and applications of temporal-focusing wide-field two-photon microscopy.pdf
Size
50.96 MB
Format
Adobe PDF
Checksum (MD5)
e17725ecbb60cbb3d32dea61a6b17416
Author(s)
So, Peter T. C.
Choi, Heejin
Yew, Yan Seng Elijah
Rowlands, Christopher
Date Issued
January 2018
Journal
Multiphoton Microscopy and Fluorescence Lifetime Imaging
Citation
So, Peter T. C., Heejin Choi, Elijah Yew, and Christopher Rowlands. “6 Principles and Applications of Temporal-Focusing Wide-Field Two-Photon Microscopy.” Multiphoton Microscopy and Fluorescence Lifetime Imaging, edited by Karsten König, 2018, pp. 103–140. © 2018 Peter T. C. So et al.
Version
Final published version
Abstract
Temporal focusing allows for rapid optically sectioned two-photon widefield microscopy. Depth sectioning is provided in a wide-field manner, without spatial focusing, by controlling the temporalwidth of femtosecond laser pulses near the focal plane. This spatial control of the temporal pulse width is achieved by diffracting the light off a grating resulting in spectral component separation and temporal broadening; these spectral components are only recombined at the focal plane to reproduce short, femtosecond pulses. Applications include (i) high speed functional imaging in the brain, (ii) fast FLIM and PLIM, (iii) cell-selective optogenetic excitation, and (iv) temporal focusing photodynamic therapy that may allow selective killing of cancer cells.
MIT Department
Massachusetts Institute of Technology. Department of Mechanical Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1515/9783110429985-008
