Ezrin regulates microvillus morphogenesis by promoting distinct activities of Eps8 proteins
Name
Zwaenepoela-2012-Ezrin regulates micr.pdf
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7.87 MB
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Adobe PDF
Checksum (MD5)
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Author(s)
Zwaenepoel, Ingrid
Da, Marcel Menezes Lyra
Maestro, Laurence Del
Formstecher, Etienne
Louvard, Daniel
Arpin, Monique
Naba, Alexandra
Date Issued
January 2012
Journal
Molecular Biology of the Cell
Publisher
American Society for Cell Biology
Citation
Zwaenepoel, I. et al. “Ezrin Regulates Microvillus Morphogenesis by Promoting Distinct Activities of Eps8 Proteins.” Molecular Biology of the Cell 23.6 (2012): 1080–1095. Web. 4 May 2012.
Version
Final published version
Abstract
The mechanisms that regulate actin filament polymerization resulting in the morphogenesis of the brush border microvilli in epithelial cells remain unknown. Eps8, the prototype of a family of proteins capable of capping and bundling actin filaments, has been shown to bundle the microvillar actin filaments. We report that Eps8L1a, a member of the Eps8 family and a novel ezrin-interacting partner, controls microvillus length through its capping activity. Depletion of Eps8L1a leads to the formation of long microvilli, whereas its overexpression has the opposite effect. We demonstrate that ezrin differentially modulates the actin-capping and -bundling activities of Eps8 and Eps8L1a during microvillus assembly. Coexpression of ezrin with Eps8 promotes the formation of membrane ruffles and tufts of microvilli, whereas expression of ezrin and Eps8L1a induces the clustering of actin-containing structures at the cell surface. These distinct morphological changes are neither observed when a mutant of ezrin defective in its binding to Eps8/Eps8L1a is coexpressed with Eps8 or Eps8L1a nor observed when ezrin is expressed with mutants of Eps8 or Eps8L1a defective in the actin-bundling or -capping activities, respectively. Our data show a synergistic effect of ezrin and Eps8 proteins in the assembly and organization of actin microvillar filaments.
MIT Department
Koch Institute for Integrative Cancer Research at MIT
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DOI of Published Version
https://doi.org/10.1091/mbc.E11-07-0588
