1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs
Name
1-s2.0-S2590018821000174-main.pdf
Description
Published version
Size
781.05 KB
Format
Adobe PDF
Checksum (MD5)
4e8ea5f395f89e45f3171948a8413fad
Author(s)
Stopfer, LE
D'Souza, AD
White, FM
Date Issued
2021
Journal
Immuno-Oncology and Technology
Publisher
Elsevier BV
Citation
Stopfer, LE, D'Souza, AD and White, FM. 2021. "1,2,3, MHC: a review of mass-spectrometry-based immunopeptidomics methods for relative and absolute quantification of pMHCs." Immuno-Oncology and Technology, 11.
Version
Final published version
Abstract
Quantitative mass-spectrometry-based methods to perform relative and absolute quantification of peptides in the immunopeptidome are growing in popularity as researchers aim to measure the dynamic nature of the peptide major histocompatibility complex repertoire and make copies-per-cell estimations of target antigens of interest. Multiple methods to carry out these experiments have been reported, each with unique advantages and limitations. This article describes existing methods and recent applications, offering guidance for improving quantitative accuracy and selecting an appropriate experimental set-up to maximize data quality and quantity.
MIT Department
Massachusetts Institute of Technology. Department of Biological Engineering
Terms of Use
Creative Commons Attribution-NonCommercial-NoDerivs License
Persistent DSpace Link
DOI of Published Version
https://doi.org/10.1016/J.IOTECH.2021.100042
