Differentiation of Gastric Helicobacter Species Using MALDI-TOF Mass Spectrometry
Name
pathogens-10-00366-v2.pdf
Description
Published version
Size
4.13 MB
Format
Unknown
Checksum (MD5)
536107ba2d4fe216922b589153bb50b8
Author(s)
Berlamont, Helena
De Witte, Chloë
De Bruyckere, Sofie
Fox, James G
Backert, Steffen
Smet, Annemieke
Boyen, Filip
Haesebrouck, Freddy
Date Issued
March 2021
Journal
Pathogens
Publisher
MDPI AG
Version
Final published version
Abstract
Gastric helicobacters (Helicobacter (H.) pylori and non-H. pylori Helicobacter species (NHPHs)) colonize the stomach of humans and/or animals. Helicobacter species identification is essential since many of them are recognized as human and/or animal pathogens. Currently, Helicobacter species can only be differentiated using molecular methods. Differentiation between NHPHs using MALDI-TOF MS has not been described before, probably because these species are poorly represented in current MALDI-TOF MS databases. Therefore, we identified 93 gastric Helicobacter isolates of 10 different Helicobacter species using MALDI-TOF MS in order to establish a more elaborate Helicobacter reference database. While the MALDI Biotyper database was not able to correctly identify any of the isolates, the in-house database correctly identified all individual mass spectra and resulted in 82% correct species identification based on the two highest log score matches (with log scores ≥2). In addition, a dendrogram was constructed using all newly created main spectrum profiles. Nine main clusters were formed, with some phylogenetically closely related Helicobacter species clustering closely together and well-defined subclusters being observed in specific species. Current results suggest that MALDI-TOF MS allows rapid differentiation between gastric Helicobacter species, provided that an extensive database is at hand and variation due to growth conditions and agar-medium-related peaks are taken into account.
MIT Department
Massachusetts Institute of Technology. Division of Comparative Medicine
Terms of Use
Creative Commons Attribution 4.0 International license
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DOI of Published Version
https://doi.org/10.3390/PATHOGENS10030366
